Composite

Part:BBa_K3134011:Design

Designed by: Shiyuan Li   Group: iGEM19_Nanjing_high-school   (2019-10-16)


T7-Cas1Cas2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We need a part which can precisely record the time in our experiments, so we think about combing T7 promoter and Cas1Cas2. Because of the presence of Cas1Cas2, it can catch the fragments when exposed to light, and by adding inducements, we can control the start of T7 promoter.


Source

Artificial synthesis and assembly.

References